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maltose binding protein mbp fusion expression vector pmal c2  (New England Biolabs)


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    New England Biolabs maltose binding protein mbp fusion expression vector pmal c2
    Maltose Binding Protein Mbp Fusion Expression Vector Pmal C2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maltose+binding+protein+mbp+fusion+expression+vector+pmal+c2/pMAL/us09296798-208-52-60
    Average 96 stars, based on 3344 article reviews
    maltose binding protein mbp fusion expression vector pmal c2 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Cloning:

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Diagnostic test for detecting anti-cashew IgE in patients
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using Pfu Turbo DNA polymerase (Stratagene Inc. Cedar Creek, T X). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containing a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Expressing:

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts (
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Diagnostic test for detecting anti-cashew IgE in patients
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using Pfu Turbo DNA polymerase (Stratagene Inc. Cedar Creek, T X). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containing a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Purification:

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Diagnostic test for detecting anti-cashew IgE in patients
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using Pfu Turbo DNA polymerase (Stratagene Inc. Cedar Creek, T X). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containing a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Modification:

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts (
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Diagnostic test for detecting anti-cashew IgE in patients
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using Pfu Turbo DNA polymerase (Stratagene Inc. Cedar Creek, T X). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containing a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Polymerase Chain Reaction:

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts (
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Diagnostic test for detecting anti-cashew IgE in patients
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using Pfu Turbo DNA polymerase (Stratagene Inc. Cedar Creek, T X). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containing a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Binding Assay:

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts (
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Diagnostic test for detecting anti-cashew IgE in patients
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, expression and purification of cDNA-encoded proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using Pfu Turbo DNA polymerase (Stratagene Inc. Cedar Creek, T X). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containing a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Purified linear epitopes from cashew nuts, nucleic acids encoding therefor, and associated methods
    Article Snippet: Similarity searches and alignments of deduced amino acid sequences were performed on Genetics Computer Group (GCG) software (Accelrys, Inc. San Diego, Calif.) using the basic local alignment search tool (BLAST) program. .. Cloning, Expression and Purification of cDNA-Encoded Proteins. cDNA coding sequences were modified by the addition of an Xba I site at 5′ end and an Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.). followed by digestion and ligated to their respective sites of the maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), containin a thrombin cleavage site. .. Competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed with cDNA/pMAL-c2 plasmids, and single colonies grown at 37° C. to an OD600 of 0.5, and induced with isopropyl-D-thiogalactopyranoside (IPTG).

    Ligation:

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts (
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′ end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.

    Article Title: Nucleic acid and allergenic polypeptides encoded thereby in cashew nuts
    Article Snippet: .. As previously described in detail (Wang et al. 2002), cDNA coding sequences were modified by the addition of a Sal I site at 5′ end and a Pst I site at 3′end by PCR using PfuTurbo DNA polymerase (Stratagene Inc., Cedar Creek, Tex.) followed by digestion with Sal I and Pst I and ligation to maltose binding protein (MBP) fusion expression vector pMAL-c2 (New England BioLabs Inc., Beverly, Mass.), into which a thrombin cleavage site had been engineered. .. For expression, competent E. coli BL21 (DE3) cells (Novagen Inc., Madison, Wis.) were transformed.



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